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Image Search Results
Journal: Glia
Article Title: GLAST-CreER T2 mediated deletion of GDNF increases brain damage and exacerbates long-term stroke outcomes after focal ischemic stroke in mouse model
doi: 10.1002/glia.23848
Figure Lengend Snippet: A) Breeding scheme to obtain tamoxifen (TAM) inducible and astrocyte-specific GLAST-GDNF−/− cKO mice. B) Gel images of genotyping. C) Experimental timeline. D) Average body weights of total, male and female of GDNFf/f and GLAST-GDNF−/− cKO mice. E) Nissl staining of brain sections showing cortical and hippocampal structure. F-G) Photographs of whole brain (F) and average brain weight (G). H-I) WB analysis of GDNF levels in the cortex and hippocampus of GDNFf/f and GLAST-GDNF−/− cKO mice using mouse anti-GDNF antibody. Summary data were obtained from the averaged values of N=5 mice for each group. J-K) WB analysis of pre- and post-synaptic proteins in the cortex (and hippocampus). Summary data were obtained from the averaged values of N=5 mice for each group.
Article Snippet: Membranes were blocked for 1 h with 5% BSA in Tris-buffered saline containing 0.1% Tween 20 (TBST) and were incubated in TBST overnight at 4 °C with first antibodies including
Techniques: Staining